Invitrogen cloning manual

TOPO® TA Cloning® Kit. Five-minute cloning of Taq polymerase-amplified PCR products. Catalog numbers (pCR ™ TOPO ® vector) K, K, KJ10, K,File Size: KB. The Donor Vector box, the Gateway® BP Clonase® II Enzyme Mix and Reagents box, and the M13 Sequencing Primers box (Boxes 1–4) contain the following items. Store the Gateway® BP Clonase® II enzyme mix at −30°C to −10°C for up to 6 months. For long-term storage, store at −85°C to −68°www.doorway.ru Size: 1MB. Several Invitrogen ™ pENTR™ vectors are available for directional TOPO cloning and direct access to the multitude of Gateway™ expression vectors. Figure 3. Strategies to build the Entry clone. The three possible methods that lead to the Entry clone are depicted: (A) BP cloning, (B) TOPO cloning, and (C) restriction enzyme and ligase www.doorway.ru Size: KB.

invitrogen-gateway-cloning-manual 1/3 Downloaded from www.doorway.ru on December 4, by guest [EPUB] Invitrogen Gateway Cloning Manual Right here, we have countless ebook invitrogen gateway cloning manual and collections to check out. We additionally come up with the money for variant types and in addition to type of the books to browse. cloning Polayes et al., pFastBac™ Dual • Two strong baculovirus promoters (PH and p10) to allow simultaneous expression of two proteins • Two large multiple cloning sites for simplified cloning Harris and Polayes, Purpose of This Manual This manual provides an overview of the Bac-to-Bac® Baculovirus Expression. TOPO® TA Cloning® Kit. Five-minute cloning of Taq polymerase-amplified PCR products. Catalog numbers (pCR ™ TOPO ® vector) K, K, KJ10, K

The easy-to-use choice for everyday cloning. Invitrogen Gateway recombination cloning technology circumvents traditional restriction enzyme based cloning limitations, enabling you to access virtually any expression system in just a few simple steps. The Donor Vector box, the Gateway® BP Clonase® II Enzyme Mix and Reagents box, and the M13 Sequencing Primers box (Boxes 1–4) contain the following items. Store the Gateway® BP Clonase® II enzyme mix at −30°C to −10°C for up to 6 months. Zero Blunt® TOPO® PCR Cloning Kit. Five-minute cloning of blunt-end PCR products. Catalog numbers K, K, KJ10, K, K, K, K, K, K, Publication Number MAN Revision B reactions. Now with 25% more TOPO! USER GUIDE For Research Use Only. Not for use in diagnostic.